Gaspar Elvira M S M, Lopes João F
Department of Chemistry, CQFB-Requimte, Faculty of Science and Technology, New University of Lisbon, Quinta da Torre, 2825-114 Caparica, Portugal.
J Chromatogr A. 2009 Apr 3;1216(14):2762-7. doi: 10.1016/j.chroma.2008.10.049. Epub 2008 Oct 17.
A new, simple, gas chromatographic method was developed for the direct analysis of 5-hydroxymethylfurfural (5-HMF), 2-furfural (2-F) and 5-methylfurfural (5-MF) in liquid and water soluble foods, using direct immersion SPME coupled to GC-FID and/or GC-TOF-MS. The fiber (DVB/CAR/PDMS) conditions were optimized: pH effect, temperature, adsorption and desorption times. The method is simple and accurate (RSD<8%), showed good recoveries (77-107%) and good limits of detection (GC-FID: 1.37 microgL(-1) for 2-F, 8.96 microgL(-1) for 5-MF, 6.52 microgL(-1) for 5-HMF; GC-TOF-MS: 0.3, 1.2 and 0.9 ngmL(-1) for 2-F, 5-MF and 5-HMF, respectively). It was applied to different commercial food matrices: honey, white, demerara, brown and yellow table sugars, and white and red balsamic vinegars. This one-step, sensitive and direct method for the analysis of furfurals will contribute to characterise and quantify their presence in the human diet.
开发了一种新的、简单的气相色谱方法，用于直接分析液体和水溶性食品中的5-羟甲基糠醛（5-HMF）、2-糠醛（2-F）和5-甲基糠醛（5-MF），该方法采用直接浸入式固相微萃取（SPME）与气相色谱-火焰离子化检测器（GC-FID）和/或气相色谱-飞行时间质谱（GC-TOF-MS）联用。对纤维（DVB/CAR/PDMS）条件进行了优化：pH值影响、温度、吸附和解吸时间。该方法简单准确（相对标准偏差<8%），回收率良好（77-107%），检测限低（GC-FID：2-F为1.37μg L-1，5-MF为8.96μg L-1，5-HMF为6.52μg L-1；GC-TOF-MS：2-F、5-MF和5-HMF分别为0.3、1.2和0.9 ng mL-1）。该方法应用于不同的商业食品基质：蜂蜜、白砂糖、德梅拉拉糖、红糖和黄糖，以及白香醋和红香醋。这种用于分析糠醛的一步式、灵敏且直接的方法将有助于表征和量化它们在人类饮食中的存在。
