Jeuring H J, Kuppers F J
J Assoc Off Anal Chem. 1980 Nov;63(6):1215-8.
Furfural (2-furaldehyde) and hydroxymethylfurfural (5-hydroxy-2-furaldehyde, HMF) are determined in brandy and honey by reverse phase high performance liquid chromatography. Brandies and other spirits are injected without sample preparation; honey is diluted with water and the solution is filtered before injection onto a reverse phase column with detection at 285 nm. The mobile phase is methanol--water (10 + 90) and the effluent flow rate is maintained at 1.0 mL/min. External standardization is used for quantitative determination. Recoveries from cognac and honey spiked at different levels ranged from 95 to 99% (furfural) and 95 to 100% (HMF). The furfural content of the brandies was also determined by the existing colorimetric method of the Bureau National Interprofessionel du Cognac. The HMF content of the honey was correlated to the results of the classic method of Winkler.
采用反相高效液相色谱法测定白兰地和蜂蜜中的糠醛（2-呋喃甲醛）和羟甲基糠醛（5-羟基-2-呋喃甲醛，HMF）。白兰地和其他烈酒无需样品制备即可直接进样；蜂蜜用水稀释，溶液过滤后再注入反相柱，于285nm处进行检测。流动相为甲醇 - 水（10 + 90），流出液流速保持在1.0 mL/min。采用外标法进行定量测定。不同加标水平的干邑白兰地和蜂蜜的回收率为95%至99%（糠醛）和95%至100%（HMF）。白兰地中的糠醛含量也采用法国国家干邑行业管理局现有的比色法进行测定。蜂蜜中的HMF含量与温克勒经典方法的结果相关。
