Filipponi Luisa, Sawant Prashant D, Fulga Florin, Nicolau Dan V
BioNanoEngineering Labs, Faculty of Engineering and Industrial Science, Swinburne University of Technology, John Street, Hawthorn, Victoria 3122, Australia.
Biosens Bioelectron. 2009 Mar 15;24(7):1850-7. doi: 10.1016/j.bios.2008.09.015. Epub 2008 Sep 24.
The rapid development of genomics and proteomics requires accelerated improvement of the microarrays density, multiplexing, readout capabilities and cost-effectiveness. The bead arrays are increasingly attractive because of their self-assembly-based fabrication, which alleviates many problems of top-down microfabrication. Here we present a simple, reliable, robust and modular technique for the fabrication of bead microarrays, which combines the directed assembling of beads in microstructures and PDMS-based replica molding. The beads are first self-assembled in pyramidal microwells fabricated by anisotropic etching of silicon substrates, then transferred on the apex of PDMS pyramids that replicate the silicon microstructures. The arrays are chemically and biochemically robust; they are spatially addressable and have the potential for being informationally addressable; and they appear to offer better readout capabilities than the classical microarrays.
基因组学和蛋白质组学的快速发展要求加快提高微阵列的密度、复用性、读出能力和成本效益。珠阵列因其基于自组装的制造方式而越来越具有吸引力，这种方式缓解了许多自上而下微制造的问题。在此，我们展示了一种用于制造珠微阵列的简单、可靠、稳健且模块化的技术，该技术将微结构中珠子的定向组装与基于聚二甲基硅氧烷（PDMS）的复制成型相结合。珠子首先在通过硅基板各向异性蚀刻制造的金字塔形微阱中自组装，然后转移到复制硅微结构的PDMS金字塔的顶点上。这些阵列在化学和生物化学方面都很稳健；它们在空间上是可寻址的，并且具有信息可寻址的潜力；而且它们似乎比传统微阵列具有更好的读出能力。
