Yu Ling, Li Chang Ming, Liu Yingshuai, Gao Jie, Wang Wei, Gan Ye
School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Dr., Singapore637457.
Lab Chip. 2009 May 7;9(9):1243-7. doi: 10.1039/b816018j. Epub 2009 Jan 20.
In this work, a dextran modified PDMS microfluidic ELISA device was fabricated. The dextran functionalization was conducted with a simple, economic and fast flow-through process in a fabricated PDMS microfluidic device, and demonstrated significant enhancement of hydrophilicity and efficient covalent immobilization of proteins on the PDMS microchannel surface. The device was used to simultaneously detect multiple important biomarker IL-5, HBsAg, and IgG, showing a limit of detection of 100 pg mL(-1) and a dynamic range of 5 orders of magnitude, which significantly improved the performance of the reported hydrophobic and plasma-treated hydrophilic PDMS flow-through immunoassay devices. The fabricated PDMS device demonstrated its capability for colorimetric detection of proteins through direct observation by human eyes. Thus, this work not only demonstrates great potential to fabricate an economical and sensitive lab-on-chip system for high throughput screening of various infectious diseases, but also provides an opportunity to develop a portable microfluidic ELISA device via human eye examination for heath point-of-care services.
在这项工作中，制备了一种葡聚糖修饰的聚二甲基硅氧烷（PDMS）微流控酶联免疫吸附测定（ELISA）装置。在制备好的PDMS微流控装置中，通过简单、经济且快速的流通法进行葡聚糖功能化，结果表明该方法显著增强了亲水性，并使蛋白质有效共价固定在PDMS微通道表面。该装置用于同时检测多种重要生物标志物白细胞介素-5（IL-5）、乙型肝炎表面抗原（HBsAg）和免疫球蛋白G（IgG），检测限为100 pg mL⁻¹，动态范围为5个数量级，这显著提高了已报道的疏水性和经血浆处理的亲水性PDMS流通免疫测定装置的性能。所制备的PDMS装置展示了通过人眼直接观察进行蛋白质比色检测的能力。因此，这项工作不仅展示了制造用于各种传染病高通量筛查的经济且灵敏的芯片实验室系统的巨大潜力，还为通过人眼检测开发用于即时医疗服务的便携式微流控ELISA装置提供了契机。
