Ko Jae-Jung, Shimizu Yoshihisa, Ikeda Kazuhiro, Kim Seog-Ku, Park Chul-Hwi, Matsui Saburo
Korea Institute of Construction Technology, 2311, Daehwa-Dong, Ilsanseo-Gu, Goyang-Si, Gyeonggi-Do 411-712, Republic of Korea.
Bioresour Technol. 2009 Feb;100(4):1622-7. doi: 10.1016/j.biortech.2008.09.029. Epub 2008 Oct 31.
This study is designed to investigate the biodegradation of high molecular weight (HMW) lignin under sulfate reducing conditions. With a continuously mesophilic operated reactor in the presence of co-substrates of cellulose, the changes in HMW lignin concentration and chemical structure were analyzed. The acid precipitable polymeric lignin (APPL) and lignin monomers, which are known as degradation by-products, were isolated and detected. The results showed that HMW lignin decreased and showed a maximum degradation capacity of 3.49 mg/l/day. APPL was confirmed as a polymeric degradation by-product and was accumulated in accordance with HMW lignin reduction. We also observed non-linear accumulation of aromatic lignin monomers such as hydrocinnamic acid. Through our experimental results, it was determined that HMW lignin, when provided with a co-substrate of cellulose, is biodegraded through production of APPL and aromatic monomers under anaerobic sulfate reducing conditions with a co-substrate of cellulose.
本研究旨在调查在硫酸盐还原条件下高分子量（HMW）木质素的生物降解情况。在存在纤维素共底物的情况下，使用连续中温运行的反应器，分析了HMW木质素浓度和化学结构的变化。分离并检测了作为降解副产物的酸沉淀聚合木质素（APPL）和木质素单体。结果表明，HMW木质素减少，最大降解能力为3.49毫克/升/天。APPL被确认为聚合降解副产物，并随着HMW木质素的减少而积累。我们还观察到了芳香族木质素单体如氢化肉桂酸的非线性积累。通过我们的实验结果确定，当提供纤维素共底物时，HMW木质素在厌氧硫酸盐还原条件下与纤维素共底物一起通过产生APPL和芳香族单体进行生物降解。
