在冷冻保存前浓缩人类精子。
Concentrating human sperm before cryopreservation.
作者信息
Kobayashi T, Kaneko S, Hara I, Park Y J, Sato H, Ohno T, Nozawa S
机构信息
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
出版信息
Andrologia. 1991 Jan-Feb;23(1):25-8. doi: 10.1111/j.1439-0272.1991.tb02487.x.
To improve the quality of cryo-preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous-step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS-II cryo-medium. The original semen (n = 32, 41 +/- 13 x 10(6)/ml, 17 +/- 11% motility) was improved by the processing to be 68 +/- 29 x 10(6)/ml, 55 +/- 19% motility. Even after the specimens were thawed, sperm concentrations were similar to those of the original semen and 39 +/- 18% motility remained with a mean survival rate of 74 +/- 7.7%. The improved sperm motility and higher survival rate resulted in better post-thaw sperm quality than those of the original semen.
为提高冷冻保存精子的质量，在冷冻前通过连续梯度密度离心法对射出的精液进行浓缩。采用液氮蒸汽和KS-II冷冻培养基简化冷冻过程。原始精液（n = 32，41±13×10⁶/ml，活力17±11%）经过处理后，精子浓度提高到68±29×10⁶/ml，活力为55±19%。即使在标本解冻后，精子浓度仍与原始精液相似，活力为39±18%，平均存活率为74±7.7%。与原始精液相比，精子活力的提高和更高的存活率导致解冻后精子质量更好。
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