Chen Huihui, Liu Sitong, Yang Fenglin, Xue Yuan, Wang Tao
Key Laboratory of Industrial Ecology and Environmental Engineering, MOE, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, PR China.
Bioresour Technol. 2009 Feb;100(4):1548-54. doi: 10.1016/j.biortech.2008.09.003. Epub 2008 Nov 5.
The simultaneous partial nitrification, ANAMMOX and denitrification (SNAD) process was validated to potentially remove ammonium and COD from wastewater in a single, oxygen-limited, non-woven rotating biological contactor (NRBC) reactor. An ammonium conversion efficiency of 79%, TN removal efficiency of 70% and COD removal efficiency of 94% were obtained with the nitrogen and COD loading rate of 0.69 kgN/m(3)d and 0.34 kg/m(3)d, respectively. Scanning electron microscopy (SEM) observation and fluorescence in situ hybridizations (FISH) analysis revealed the existence of the dominant groups of bacteria. As a result, the aerobic ammonia-oxidizing bacteria (AOB), with a spot of aerobic heterotrophic bacteria were mainly distributed in the aerobic outer part of the biofilm. However, ANAMMOX bacteria with denitrifying bacteria were present and active in the anaerobic inner part of the SNAD biofilm. These bacteria were found to exist in a dynamic equilibrium to achieve simultaneous nitrogen and COD removal in NRBC system.
同步亚硝化、厌氧氨氧化和反硝化（SNAD）工艺经证实可在单个限氧的非织造旋转生物接触器（NRBC）反应器中从废水中去除铵和化学需氧量（COD）。在氮负荷率和COD负荷率分别为0.69 kgN/m³·d和0.34 kg/m³·d的情况下，铵转化效率达到79%，总氮去除效率达到70%，COD去除效率达到94%。扫描电子显微镜（SEM）观察和荧光原位杂交（FISH）分析揭示了优势菌群的存在。结果表明，好氧氨氧化细菌（AOB）以及少量好氧异养细菌主要分布在生物膜的好氧外层。然而，厌氧氨氧化细菌和反硝化细菌存在于SNAD生物膜的厌氧内层并具有活性。发现这些细菌以动态平衡的状态存在，从而在NRBC系统中实现氮和COD的同步去除。
