CASTOR和POLLUX这两个双子离子通道编码基因在植物根系共生进化中的古老性与功能，它们是植物根系共生进化的关键基因。
Antiquity and function of CASTOR and POLLUX, the twin ion channel-encoding genes key to the evolution of root symbioses in plants.
作者信息
Chen Caiyan, Fan Cui, Gao Muqiang, Zhu Hongyan
机构信息
Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky 40546, USA.
出版信息
Plant Physiol. 2009 Jan;149(1):306-17. doi: 10.1104/pp.108.131540. Epub 2008 Oct 31.
Abstract
Root symbioses with arbuscular mycorrhizal fungi and rhizobial bacteria share a common signaling pathway in legumes. Among the common symbiosis genes are CASTOR and POLLUX, the twin homologous genes in Lotus japonicus that encode putative ion channel proteins. Here, we show that the orthologs of CASTOR and POLLUX are ubiquitously present and highly conserved in both legumes and nonlegumes. Using rice (Oryza sativa) as a study system, we employ reverse genetic tools (knockout mutants and RNA interference) to demonstrate that Os-CASTOR and Os-POLLUX are indispensable for mycorrhizal symbiosis in rice. Furthermore, a cross-species complementation test indicates that Os-POLLUX can restore nodulation, but not rhizobial infection, to a Medicago truncatula dmi1 mutant.
摘要
豆科植物与丛枝菌根真菌和根瘤菌的根系共生共享一条共同的信号通路。在常见的共生基因中，有CASTOR和POLLUX，它们是百脉根中的一对同源基因，编码假定的离子通道蛋白。在这里，我们表明CASTOR和POLLUX的直系同源基因在豆科植物和非豆科植物中普遍存在且高度保守。以水稻（Oryza sativa）为研究系统，我们利用反向遗传学工具（敲除突变体和RNA干扰）证明Os-CASTOR和Os-POLLUX对水稻菌根共生必不可少。此外，一项跨物种互补试验表明，Os-POLLUX可以恢复蒺藜苜蓿dmi1突变体的结瘤能力，但不能恢复根瘤菌感染能力。
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