将双链RNA注射到雌性埃及伊蚊体内。
Injection of dsRNA into female A. aegypti mosquitos.
作者信息
Luna Brian M, Juhn Jennifer, James Anthony A A
机构信息
Department of Molecular Biology and Biochemistry, University of California, Irvine, USA.
出版信息
J Vis Exp. 2007(5):215. doi: 10.3791/215. Epub 2007 Jul 4.
Abstract
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
摘要
反向遗传学方法已被证明对于确定蚊子中哪些基因是对媒介病原体抗性的基础极为有用。本视频方案展示了詹姆斯实验室用于将双链RNA注射到携带登革热病毒的雌性埃及伊蚊体内的一种方法。视频展示了校准注射针、操作注射装置以及将双链RNA注射到胸部的技术。
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