酵母交配型细胞融合检测

Cell fusion assays for yeast mating pairs.

作者信息

Grote Eric

机构信息

Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

出版信息

Methods Mol Biol. 2008;475:165-96. doi: 10.1007/978-1-59745-250-2_10.

DOI:10.1007/978-1-59745-250-2_10

PMID:18979244

Abstract

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

摘要

酵母交配为发现真核细胞融合的基本机制提供了一个易于研究的遗传系统。尽管与信息素信号传导和极性生长相关的酵母交配方面已得到深入研究，但融合本身却知之甚少。本章介绍了测量酵母细胞融合总体效率以及监测融合过程各个阶段的方法，这些阶段包括细胞壁重塑、质膜融合和核融合。