[糖皮质激素性股骨头坏死骨组织中骨保护素mRNA/核因子κB受体活化因子配体mRNA表达]

[Osteoprotegerin mRNA/receptor activator of NF-kappaB ligand mRNA expressions in bone tissues of glucocorticoid-induced osteonecrosis of the femoral head].

作者信息

Wang Jianzhong, Wang Kunzheng, Shi Zhibin, Zhang Mingyu

机构信息

Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an Shaanxi 710004, PR China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008 Oct;22(10):1161-4.

PMID:18979868

Abstract

OBJECTIVE

To investigate the expression levels of osteoprotegerin (OPG) and receptor activator of NF-kappaB ligand (RANKL) mRNAs in bone tissues of the femoral head of the patients suffering glucocorticoid-induced osteonecrosis of the femoral head (ONFH), and to discuss the relationship between OPG/RANKL and ONFH.

METHODS

Between March 2007 and March 2008, bone tissues of the femoral head were collected as the experimental material from 35 patients suffering ONFH (experimental group) and from 21 patients suffering fracture of femoral neck (control group). The ratio of men to women in both groups was 4 : 3, whose age was 41-70 years old (55.34 on average in the experimental group and 55.33 on average in the control group). The experimental group received over 3 weeks' glucocorticoid treatment or more than 1 week's high-dose glucocorticoid treatment in recent 2 years, while the control group never received more than 1 week's hormone treatment. In the two groups, the microstructure of bone tissues of the femoral head was detected by HE staining and the bone tissue total RNA was extracted, and then the expression levels of OPG mRNA and RANKL mRNA were examined by real-time quantitative PCR (RTQ-PCR) for each sample.

RESULTS

HE staining: bone trabeculae and bone units were replaced by interrupted bone fragments, which were surrounded by many inflammatory granulation tissues and few osteocytes were seen in bone lacunae in the experimental group. In the control group, bone trabeculae and bone units were made by complete lamellar bones which surrounded blood vessels and osteocytes were seen in lacunae. RTQ-PCR testing: in the experimental group, OPG mRNA and RANKL mRNA were 1.35 +/- 0.42 and 4.36 +/- 1.35, respectively, while in the control group they were 1.78 +/- 0.63 and 3.49 +/- 1.02, respectively. The expression level of OPG mRNA in the experimental group was significantly lower than that in the control group, and the expression level of RANKL mRNA of the former was significantly higher than the latter. The OPG mRNA/ RANKL mRNA ratio in the experiment group (0.34 +/- 0.16) was significantly lower than that in the control group (0.54 +/- 0.20), and there was significant difference (P < 0.05).

CONCLUSION

The glucocorticoid-induced ONFH may be related to the expression levels of OPG mRNA/RANKL mRNA in bone tissues.

摘要

目的

探讨糖皮质激素性股骨头坏死（ONFH）患者股骨头骨组织中骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）mRNA的表达水平，并探讨OPG/RANKL与ONFH之间的关系。

方法

2007年3月至2008年3月，收集35例ONFH患者（实验组）和21例股骨颈骨折患者（对照组）的股骨头骨组织作为实验材料。两组男女比例均为4∶3，年龄41 - 70岁（实验组平均55.34岁，对照组平均55.33岁）。实验组在近2年内接受过3周以上糖皮质激素治疗或1周以上大剂量糖皮质激素治疗，对照组从未接受过1周以上激素治疗。两组均采用HE染色检测股骨头骨组织的微观结构，提取骨组织总RNA，然后用实时定量PCR（RTQ-PCR）检测各样本中OPG mRNA和RANKL mRNA的表达水平。

结果

HE染色：实验组骨小梁和骨单位被中断的骨碎片取代，周围有许多炎性肉芽组织，骨陷窝内可见少量骨细胞。对照组骨小梁和骨单位由完整的板层骨构成，包绕血管，陷窝内可见骨细胞。RTQ-PCR检测：实验组OPG mRNA和RANKL mRNA分别为1.35±0.42和4.36±1.35，对照组分别为1.78±0.63和3.49±1.02。实验组OPG mRNA表达水平明显低于对照组，RANKL mRNA表达水平明显高于对照组。实验组OPG mRNA/RANKL mRNA比值（0.34±0.16）明显低于对照组（0.54±0.20），差异有统计学意义（P < 0.05）。