Otake Takamitsu, Aoyagi Yoshie, Numata Masahiko, Yarita Takashi
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8563, Japan.
J AOAC Int. 2008 Sep-Oct;91(5):1124-9.
The efficiency of microwave-assisted extraction (MAE) was evaluated for the analysis of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in fish. An isotope dilution method was used for quantification via analysis of the samples by gas chromatography and mass spectrometry. MAE solvent, temperature, and time were optimized, and observed concentrations were compared. The MAE results were also compared to those of other extraction techniques (Soxhlet extraction, pressurized liquid extraction, saponification, and homogenization). Concentrations of PCBs and OCPs obtained by MAE at 120 degrees C for 10 min were comparable to those by the other techniques. The results suggest that MAE can be used for the analysis of PCBs and OCPs in fish.
评估了微波辅助萃取（MAE）用于分析鱼类中多氯联苯（PCBs）和有机氯农药（OCPs）的效率。采用同位素稀释法，通过气相色谱和质谱分析样品进行定量。对MAE的溶剂、温度和时间进行了优化，并比较了观察到的浓度。还将MAE的结果与其他萃取技术（索氏萃取、加压液体萃取、皂化和匀浆法）的结果进行了比较。在120℃下MAE萃取10分钟得到的PCBs和OCPs浓度与其他技术得到的浓度相当。结果表明，MAE可用于分析鱼类中的PCBs和OCPs。
