Zheng Ping-Pin, Severijnen Lies-Anne, van der Weiden Marcel, Willemsen Rob, Kros Johan M
Department of Pathology, Erasmus Medical Center, JNI Room 230-c, Dr Molewaterplein 50, PO Box 1738, 3000 DR Rotterdam, The Netherlands.
Cardiovasc Res. 2009 Feb 1;81(2):362-9. doi: 10.1093/cvr/cvn294. Epub 2008 Nov 3.
We explored the in vivo effects of knockdown of caldesmon on vascular development in zebrafish.
We investigated the effects of caldesmon knockdown on the vascular development in a zebrafish model with special attention for the trunk and head vessels including the aortic arches. We examined the developing fishes at various time points. The vascular abnormalities observed in the caldesmon morphants were morphologically and functionally characterized in detail in fixed and living embryos. The knockdown of caldesmon caused serious defects in vasculogenesis and angiogenesis in zebrafish morphants, and the vascular integrity and blood circulation were concomitantly impaired.
The data provide the first functional assessment of the role of caldesmon in vascular development in vivo, indicating that this molecule plays a crucial role in vasculogenesis and angiogenesis in vivo. Interfering with caldesmon opens new therapeutic avenues for anti-angiogenesis in cancer and ischaemic cardiovascular disease.
我们探究了抑制钙调蛋白对斑马鱼血管发育的体内影响。
我们在斑马鱼模型中研究了钙调蛋白抑制对血管发育的影响，特别关注包括主动脉弓在内的躯干和头部血管。我们在不同时间点检查发育中的鱼类。在固定和活体胚胎中详细表征了钙调蛋白 morphants 中观察到的血管异常的形态和功能特征。钙调蛋白的抑制导致斑马鱼 morphants 中血管生成和血管新生出现严重缺陷，同时血管完整性和血液循环也受到损害。
这些数据首次对钙调蛋白在体内血管发育中的作用进行了功能评估，表明该分子在体内血管生成和血管新生中起关键作用。干扰钙调蛋白为癌症和缺血性心血管疾病的抗血管生成开辟了新的治疗途径。
