Dehard Sandrine, Bernaerts Frederique, Peeters Dominique, Detilleux Johann, McEntee Kathleen, Day Michael J, Clercx Cecile
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium.
J Am Anim Hosp Assoc. 2008 Nov-Dec;44(6):285-94. doi: 10.5326/0440285.
Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease. Bronchoalveolar lavage fluid from healthy cats was also examined. With MSP, cell lysis was more frequently observed, and cellular distribution was more heterogeneous throughout the slide. When samples from healthy and diseased animals were considered together, a significantly greater percentage of neutrophils was seen on CP than on MSP slides (P<0.002). Cytospin preparations were considered of better quality in all individual comparisons. Cytospin preparation is advised in the evaluation of BALF with low total cell count. When only MSPs are evaluated, clinicians should be aware that differential neutrophil counts may underestimate the counts found on CP slides.
在患有炎性或感染性下呼吸道疾病的客户拥有的犬猫中,研究了细胞离心涂片制备(CP)或手工涂片制备(MSP)的颗粒细胞后支气管肺泡灌洗(BALF)的细胞学解释差异。还检查了健康猫的支气管肺泡灌洗。采用MSP时,更频繁地观察到细胞溶解,并且细胞在整个载玻片上的分布更不均匀。当将来自健康和患病动物的样本一起考虑时,CP涂片上的中性粒细胞百分比显著高于MSP涂片(P<0.002)。在所有个体比较中,细胞离心涂片制备的质量被认为更好。对于总细胞数较低的BALF评估,建议采用细胞离心涂片制备。当仅评估MSP时,临床医生应意识到中性粒细胞分类计数可能低估CP涂片上的计数。