Nafe Laura A, DeClue Amy E, Reinero Carol R
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
J Feline Med Surg. 2011 Feb;13(2):94-100. doi: 10.1016/j.jfms.2010.09.017. Epub 2010 Dec 28.
Bronchoalveolar lavage fluid (BALF) collection is a valuable respiratory diagnostic procedure in cats. This study evaluated effects of BALF storage on total nucleated cell counts (TNCCs) and differential cell counts (DCC), cell morphology, and cytological diagnosis. Forty-five research cats with neutrophilic, eosinophilic, and mixed inflammation, and healthy controls were enrolled. BALF samples were processed within 1h (baseline) or stored at 4°C (4C24) or room temperature (RT24) for 24h, or 4°C (4C48) or room temperature (RT48) for 48h before processing. Stored BALF at RT48 had decreased TNCC compared to baseline. The RT24 and RT48 samples had greater eosinophil % and the RT24, 4C48, and RT48 samples had decreased neutrophil % compared with baseline. Cellular morphology deteriorated in all stored samples. Storage resulted in a change in cytological diagnosis in up to 57% of stored samples. We conclude that cytological analysis of BALF in cats should be performed promptly for optimal results.
支气管肺泡灌洗术（BALF）采集是猫科动物中一项有价值的呼吸诊断程序。本研究评估了BALF储存对总核细胞计数（TNCCs）、分类细胞计数（DCC）、细胞形态和细胞学诊断的影响。纳入了45只患有嗜中性、嗜酸性和混合性炎症的研究用猫以及健康对照。BALF样本在1小时内（基线）进行处理，或在处理前于4°C（4C24）或室温（RT24）储存24小时，或于4°C（4C48）或室温（RT48）储存48小时。与基线相比，RT48储存的BALF的TNCC有所下降。与基线相比，RT24和RT48样本的嗜酸性粒细胞百分比更高，而RT24、4C48和RT48样本的嗜中性粒细胞百分比有所下降。所有储存样本的细胞形态均恶化。储存导致高达57%的储存样本的细胞学诊断发生变化。我们得出结论，猫BALF的细胞学分析应及时进行以获得最佳结果。
