一氧化氮合酶缺陷的克氏锥虫感染小鼠肠道扩张的磁共振成像研究
A magnetic resonance imaging study of intestinal dilation in Trypanosoma cruzi-infected mice deficient in nitric oxide synthase.
作者信息
Ny Lars, Li Hua, Mukherjee Shankar, Persson Katarina, Holmqvist Bo, Zhao Dazhi, Shtutin Vitaliy, Huang Huan, Weiss Louis M, Machado Fabiana S, Factor Stephen M, Chan John, Tanowitz Herbert B, Jelicks Linda A
机构信息
Department of Oncology, Sahlgrenska University Hospital, SE-413 45 Göteborg, Sweden.
出版信息
Am J Trop Med Hyg. 2008 Nov;79(5):760-7.
Infection with Trypanosoma cruzi causes megasyndromes of the gastrointestinal (GI) tract. We used magnetic resonance imaging (MRI) to monitor alterations in the GI tract of T. cruzi-infected mice, and to assess the role of nitric oxide (NO) in the development of intestinal dilation. Brazil strain-infected C57BL/6 wild-type (WT) mice exhibited dilatation of the intestines by 30 days post-infection. Average intestine lumen diameter increased by 72%. Levels of intestinal NO synthase (NOS) isoforms, NOS2 and NOS3, were elevated in infected WT mice. Inflammation and ganglionitis were observed in all infected mice. Intestinal dilation was observed in infected WT, NOS1, NOS2, and NOS3 null mice. This study demonstrates that MRI is a useful tool to monitor intestinal dilation in living mice and that these alterations may begin during acute infection. Furthermore, our data strongly suggests that NO may not be the sole contributor to intestinal dysfunction resulting from this infection.
克氏锥虫感染会引发胃肠道的巨大综合征。我们使用磁共振成像（MRI）来监测克氏锥虫感染小鼠胃肠道的变化，并评估一氧化氮（NO）在肠道扩张发展中的作用。感染巴西株的C57BL/6野生型（WT）小鼠在感染后30天出现肠道扩张。平均肠腔直径增加了72%。感染的WT小鼠肠道中NO合酶（NOS）同工型NOS2和NOS3的水平升高。在所有感染小鼠中均观察到炎症和神经节炎。在感染的WT、NOS1、NOS2和NOS3基因敲除小鼠中均观察到肠道扩张。本研究表明，MRI是监测活体小鼠肠道扩张的有用工具，且这些变化可能在急性感染期间就已开始。此外，我们的数据强烈表明，NO可能不是这种感染导致肠道功能障碍的唯一因素。
Zotero 插件