Fukamizu A, Hatae T, Kon Y, Sugimura M, Hasegawa T, Yokoyama M, Nomura T, Katsuki M, Murakami K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Biochem J. 1991 Sep 1;278 ( Pt 2)(Pt 2):601-3. doi: 10.1042/bj2780601.
To examine whether expression of human renin in the transgenic mouse kidney is regulated in a cell-specific manner, we have characterized monoclonal antibodies against human renin and determined the renin-production site by immunohistochemistry. By using a monoclonal antibody specific for human renin, A6-11-6, we demonstrated that human renin in the transgenic mouse kidney is localized to the juxtaglomerular cells of afferent arterioles.
为了研究转基因小鼠肾脏中人类肾素的表达是否以细胞特异性方式受到调控，我们鉴定了抗人类肾素的单克隆抗体，并通过免疫组织化学确定了肾素产生部位。通过使用对人类肾素特异的单克隆抗体A6 - 11 - 6，我们证明转基因小鼠肾脏中的人类肾素定位于入球小动脉的球旁细胞。
