Wei Juan, Zhang Xiao-lan, Yao Dong-mei, Huo Xiao-xia, Shen Jian-gang, Dun Zhi-na
Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Zhonghua Gan Zang Bing Za Zhi. 2008 Oct;16(10):757-61.
To investigate the effects of FAK-related non-kinase (FRNK) on expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) mRNA and tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in rat hepatic stellate cells (HSC).
Using in vitro cell culture technique, FRNK plasmids were transfected into HSC mediated by cationic liposome. Type I collagen synthesis capability in HSC was examined by 3H-Pro incorporation assay. The levels of FRNK in HSC were assayed by Western blot, and the expressions of MMP-2 and TIMP-2 were assayed by RT-PCR on mRNA levels.
The exposure of HSC to FRNK caused the expression of FRNK protein to be up-regulated, and the FRNK protein contents reached the highest point at 48 h after the transfection, P less than 0.05. The expressions of MMP-2 mRNA were up-regulated by FRNK; the expressions of TIMP-2 mRNA were down-regulated by FRNK; the ratios of MMP-2 mRNA/TIMP-2 mRNA were enhanced by FRNK.
After FRNK was transfected, the capability of type I collagen synthesis in HSC was inhibited, which may be related to the up-regulation of MMP-2 mRNA/TIMP-2 mRNA.
研究黏着斑激酶相关非激酶（FRNK）对大鼠肝星状细胞（HSC）中Ⅰ型胶原、基质金属蛋白酶-2（MMP-2）mRNA及金属蛋白酶组织抑制剂-2（TIMP-2）mRNA表达的影响。
采用体外细胞培养技术，以阳离子脂质体介导将FRNK质粒转染至HSC。通过3H-脯氨酸掺入法检测HSC中Ⅰ型胶原合成能力。采用蛋白质免疫印迹法检测HSC中FRNK水平，采用逆转录-聚合酶链反应（RT-PCR）检测mRNA水平的MMP-2和TIMP-2表达。
HSC暴露于FRNK后，FRNK蛋白表达上调，转染后48 h时FRNK蛋白含量达最高点，P<0.05。FRNK上调MMP-2 mRNA表达；FRNK下调TIMP-2 mRNA表达；FRNK提高MMP-2 mRNA/TIMP-2 mRNA比值。
转染FRNK后，HSC中Ⅰ型胶原合成能力受到抑制，这可能与MMP-2 mRNA/TIMP-2 mRNA上调有关。
