Lambert S, Muyldermans S, Baldwin J, Kilner J, Ibel K, Wijns L
Liverpool Polytechnic, U.K.
Biochem Biophys Res Commun. 1991 Sep 16;179(2):810-6. doi: 10.1016/0006-291x(91)91889-k.
Neutron scattering data establish that the radius of gyration of the DNA in chicken erythrocyte chromatosome particles is significantly higher, by about 0.3 nm, than the radius of gyration of the DNA in the core particle. Corresponding information of the radius of gyration of the protein component in the chromatosomes (3.75 nm) indicated an enlargement, compared to the radius of gyration of the octamer of histone proteins both in core particles and in the histone octamer stabilised in 2 M NaCl (3.25 nm). From the latter data, we could calculate the distance in the chromatosome between the centre of mass of the linker histone and the histone octamer as 5.5 nm. These results impose severe limitations for the organisation of the 22 bp extra DNA and the possible location of H1/H5 in the chromatosome, implying that the H1/H5 is close to the centre turn of the core particle DNA.
中子散射数据表明，鸡红细胞染色质体颗粒中DNA的回转半径比核心颗粒中DNA的回转半径显著更高，约高0.3纳米。染色质体中蛋白质组分的回转半径（3.75纳米）的相应信息表明，与核心颗粒中以及在2 M NaCl中稳定的组蛋白八聚体（3.25纳米）的回转半径相比有所增大。根据后一组数据，我们可以计算出连接组蛋白的质心与组蛋白八聚体在染色质体中的距离为5.5纳米。这些结果对22 bp额外DNA的组织以及H1/H5在染色质体中的可能位置施加了严格限制，这意味着H1/H5靠近核心颗粒DNA的中心转角。
