Jenkins T M, Gorrell T E, Müller M, Weitzman P D
Rockefeller University, New York, NY 10021.
Biochem Biophys Res Commun. 1991 Sep 16;179(2):892-6. doi: 10.1016/0006-291x(91)91902-o.
Succinate thiokinase displays a diversity of nucleotide specificity and molecular size throughout Nature. Eukaryotes and Gram-positive bacteria possess distinct 'small' (dimeric) thiokinase enzymes which are specific for adenine (ADP) or guanine (GDP) nucleotides, whereas Gram-negative bacteria contain a single 'large' (tetrameric) enzyme which utilizes both nucleotides. Succinate thiokinase activities, both ADP- and GDP-dependent, were shown to be hydrogenosomal in Tritrichomonas foetus and Trichomonas vaginalis. Surprisingly, the 'small' enzyme was found in T. foetus whereas T. vaginalis contained a 'large' enzyme.
琥珀酸硫激酶在自然界中呈现出核苷酸特异性和分子大小的多样性。真核生物和革兰氏阳性菌拥有独特的“小”（二聚体）硫激酶酶，它们分别对腺嘌呤（ADP）或鸟嘌呤（GDP）核苷酸具有特异性，而革兰氏阴性菌含有一种单一的“大”（四聚体）酶，可利用这两种核苷酸。在胎儿三毛滴虫和阴道毛滴虫中，琥珀酸硫激酶活性，包括依赖ADP和GDP的活性，均显示存在于氢化酶体中。令人惊讶的是，在胎儿三毛滴虫中发现了“小”酶，而阴道毛滴虫中含有“大”酶。
