Binding enhancement of antigen-functionalized PEGylated gold nanoparticles onto antibody-immobilized surface by increasing the functionalized antigen using alpha-sulfanyl-omega-amino-PEG.

作者信息

Yoshimoto Keitaro, Hoshino Yuki, Ishii Takehiko, Nagasaki Yukio

机构信息

Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, Japan.

出版信息

Chem Commun (Camb). 2008 Nov 14(42):5369-71. doi: 10.1039/b811818c. Epub 2008 Sep 16.

DOI:10.1039/b811818c

PMID:18985213

Abstract

We established a technique for constructing PEGylated gold nanoparticles (GNP) that have small compounds with almost complete functionalities on their surfaces using a newly synthesized hetero-telechelic poly(ethylene glycol) (PEG), and their association/dissociation behavior on an antibody-immobilized surface was evaluated using a surface plasmon resonance (SPR) sensor.

摘要