Lee Woochang, Oh Byung-Keun, Kim Yong-Wan, Choi Jeong-Woo
Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea.
J Nanosci Nanotechnol. 2006 Nov;6(11):3521-5.
In the immunoassay based on surface plasmon resonance (SPR) system, the signal enhancement was done by means of the conjugate of gold (Au) nanoparticle-antibody fragment. Antibody fragment was prepared for the improved immobilization based on Au-thiol interaction. Through the ellipsometric analysis on surface, the conjugation between Au and antibody fragment was performed in the oriented manner. The optimal fabrication conditions such as concentration and incubation time were determined for the constant size of the fabricated nanoparticle-antibody conjugate. Through the plot of SPR angle difference versus antigen concentration, the linear correlation was achieved, of which the detection limit was 100 fg/ml.
在基于表面等离子体共振（SPR）系统的免疫分析中，通过金（Au）纳米颗粒 - 抗体片段共轭物实现信号增强。基于金 - 硫醇相互作用制备抗体片段以实现更好的固定化。通过表面椭偏分析，以定向方式进行金与抗体片段之间的共轭。针对制备的纳米颗粒 - 抗体共轭物的恒定尺寸，确定了诸如浓度和孵育时间等最佳制备条件。通过绘制SPR角度差与抗原浓度的关系图，实现了线性相关，其检测限为100 fg/ml。
