Graz Marcin, Jarosz-Wilkołazka Anna, Pawlikowska-Pawlega Bozena
Department of Biochemistry, Maria Curie-Sklodowska University, Sklodowska Place 3, 20-031 Lublin, Poland.
Biometals. 2009 Jun;22(3):401-10. doi: 10.1007/s10534-008-9176-1. Epub 2008 Nov 5.
The ability of Abortiporus biennis to tolerate and solubilize toxic metal oxides (Cu(2)O, Al(2)O(3), ZnO, CuFe(2)O(4)Zn, CdO, and MnO(2)) incorporated into agar media was investigated and the growth rate, oxalic acid secretion, and mycelial morphology were monitored. Among the tested metal oxides, formation of clear zones underneath the mycelium growing on Cu(2)O- and ZnO-amended plates was observed. ZnO, CdO and Cu(2)O caused the highest rate of fungal growth inhibition. An increased level of oxalic acid concentration was detected as a response of A. biennis to the presence of Cu(2)O, MnO(2), ZnO and CuFe(2)O(4)Zn in growth medium. The oxalate oxidase (OXO) was found to be responsible for oxalic acid degradation in A. biennis cultivated in metal-amended media. An increased level of OXO was observed in media amended with Cu(2)O, ZnO and MnO(2). Confocal microscopy used in this study revealed changes in mycelial morphology which appeared as increased hyphal branching, increased septation and increased spore number.
研究了双孢黄多孔菌耐受和溶解掺入琼脂培养基中的有毒金属氧化物（Cu₂O、Al₂O₃、ZnO、CuFe₂O₄Zn、CdO和MnO₂）的能力，并监测了其生长速率、草酸分泌和菌丝形态。在测试的金属氧化物中，观察到在添加了Cu₂O和ZnO的平板上生长的菌丝体下方形成了透明圈。ZnO、CdO和Cu₂O对真菌生长的抑制率最高。检测到双孢黄多孔菌对生长培养基中Cu₂O、MnO₂、ZnO和CuFe₂O₄Zn的存在作出反应，草酸浓度有所增加。发现草酸氧化酶（OXO）负责在添加金属的培养基中培养的双孢黄多孔菌中草酸的降解。在用Cu₂O、ZnO和MnO₂改良的培养基中观察到OXO水平增加。本研究中使用的共聚焦显微镜揭示了菌丝形态的变化，表现为菌丝分支增加、隔膜增加和孢子数量增加。
