Rudholm Tobias, Hellstrom Per-Mikael, Theodorsson Elvar, Campbell Colin-Allan, McLean Peter-Geoffrey, Naslund Erik
Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
World J Gastroenterol. 2008 Oct 28;14(40):6180-7. doi: 10.3748/wjg.14.6180.
To evaluate measurements of intragastric pH with the Bravo capsule system over a prolonged time.
A Bravo capsule was placed inside the rat gastric body and pH was studied for periods up to five consecutive days. For comparison, a gastric fistula model was used. Effects of ghrelin and esomeprazole, with or without pentagastrin, on gastric pH were studied. In addition, effects of esomeprazole on plasma ghrelin, gastrin and somatostatin were analyzed.
All rats recovered after surgery. The average 24-h pH during free feeding was 2.3 +/- 0.1 (n = 20) with a variation of 18% +/- 6% over 5 d. Ghrelin, 2400 pmol/kg, t.i.d. increased pH from 1.7 +/- 0.1 to 3.1 +/- 0.3 (P < 0.01) as recorded with the Bravo system. After esomeprazole (1 mg/kg, 3 mg/kg and 5 mg/kg) there was a dose-dependent pH increase of maximally 3.4 +/- 0.1, with day-to-day variation over the entire period of 8% +/- 3%. The fistula and pH studies generated similar results. Acid inhibition with esomeprazole increased plasma ghrelin from 10 +/- 2 pmol/L to 65 +/- 26 pmol/L (P < 0.001), and somatostatin from 10 +/- 2 pmol/L to 67 +/- 18 pmol/L (P < 0.001).
pH measurements with the Bravo capsule are reliable, and comparable to those of the gastric fistula model. The Bravo system optimizes accurate intragastric pH monitoring over prolonged periods and allows both short- and long-term evaluation of effects of drugs and hormones.
通过Bravo胶囊系统对胃内pH值进行长时间评估。
将一个Bravo胶囊置于大鼠胃体内部，对pH值进行连续长达五天的研究。为作比较，采用了胃瘘模型。研究了胃饥饿素和埃索美拉唑在有无五肽胃泌素情况下对胃pH值的影响。此外，还分析了埃索美拉唑对血浆胃饥饿素、胃泌素和生长抑素的影响。
所有大鼠术后均恢复。自由进食期间的平均24小时pH值为2.3±0.1（n = 20），5天内变化幅度为18%±6%。胃饥饿素，2400 pmol/kg，每日三次，使pH值从1.7±0.1升至3.1±0.3（P < 0.01），这是用Bravo系统记录的结果。给予埃索美拉唑（1 mg/kg、3 mg/kg和5 mg/kg）后，pH值呈剂量依赖性升高，最大升高至3.4±0.1，在整个期间内每日变化幅度为8%±3%。瘘管和pH值研究得出了相似结果。埃索美拉唑抑制胃酸使血浆胃饥饿素从10±2 pmol/L升至65±26 pmol/L（P < 0.001），生长抑素从10±2 pmol/L升至67±18 pmol/L（P < 0.001）。
用Bravo胶囊进行pH值测量是可靠的，且与胃瘘模型的测量结果相当。Bravo系统优化了长时间内胃内pH值的精确监测，并允许对药物和激素的作用进行短期和长期评估。
