Schmucker D L, Wang R K, Snyder D, Strobel H, Marti U
Cell Biology and Aging Section VA Medical Center, San Francisco, CA 94121.
J Gerontol. 1991 Jan;46(1):B23-7. doi: 10.1093/geronj/46.1.b23.
Caloric restriction (CR) extends life span and retards the onset of physiological changes and pathologies associated with aging, but the underlying mechanisms remain unresolved. This study demonstrates that CR postpones the documented age-related declines in and/or enhances the activity and microsomal concentration of several liver monooxygenases in male rats, i.e., NADPH cytochrome P-450 reductase, total cytochromes P-450. However, the relative concentration of cytochrome P-450b+C did not exhibit statistically significant changes, whereas another isozyme, the male specific P-450h, declined significantly in both ad libitum-fed and CR rats as a function of increasing age. While CR appears to retard age-associated changes in certain liver enzymes, this effect is by no means universal. The hepatic monooxygenases constitute a well-characterized enzyme system in which to examine the perturbation of the aging process by CR.
热量限制（CR）可延长寿命，并延缓与衰老相关的生理变化和病理状态的出现，但其潜在机制仍未得到解决。本研究表明，热量限制可推迟雄性大鼠中已记录的与年龄相关的几种肝脏单加氧酶活性下降和/或提高其微粒体浓度，即NADPH细胞色素P-450还原酶、总细胞色素P-450。然而，细胞色素P-450b+C的相对浓度未表现出统计学上的显著变化，而另一种同工酶，即雄性特异性P-450h，在自由采食和热量限制的大鼠中均随着年龄增长而显著下降。虽然热量限制似乎可延缓某些肝脏酶与年龄相关的变化，但这种作用并非普遍存在。肝脏单加氧酶构成了一个特征明确的酶系统，可用于研究热量限制对衰老过程的干扰。
