Mishin V M, Grishanova A Iu, Liakhovich V V
Biokhimiia. 1984 Apr;49(4):686-91.
The reconstitution of microsomal membrane monooxygenase system with variable contents of the hydroxylating chain enzymatic components was carried out. It was found that during self-assembly of microsomal membranes solubilized with 4% sodium cholate and gel filtration through Sephadex LH-20 in the presence of isolated microsomal enzymes, two forms of cytochrome P-450, i. e. phenobarbital- and 3-methylcholantrene-induced ones, and NADPH-cytochrome P-450 reductase, the exogenous enzymes are incorporated into the microsomal membrane matrices of control and methyl-cholantrene-treated animals. In the membranes reconstituted from the microsomes of the methylcholantrene-induced animals the catalytic activity of cytochrome P-448 in the metabolism of benz(a)pyrene at varying cytochrome P-448 and NADPH-cytochrome P-450 reductase contents were investigated.
进行了具有不同含量羟化链酶成分的微粒体膜单加氧酶系统的重组。发现在用4%胆酸钠溶解的微粒体膜自组装过程中，以及在分离的微粒体酶存在下通过葡聚糖LH - 20进行凝胶过滤时，两种形式的细胞色素P - 450，即苯巴比妥诱导型和3 - 甲基胆蒽诱导型，以及NADPH - 细胞色素P - 450还原酶，这些外源酶被整合到对照动物和甲基胆蒽处理动物的微粒体膜基质中。在由甲基胆蒽诱导动物的微粒体制备的重组膜中，研究了在不同细胞色素P - 448和NADPH - 细胞色素P - 450还原酶含量下，细胞色素P - 448在苯并(a)芘代谢中的催化活性。
