Olson V L, Hansing R L, McClary D O
Can J Microbiol. 1977 Feb;23(2):166-74. doi: 10.1139/m77-024.
Comparative studies were made on the destructive effects of certain basic proteins on a strain of Candida albicans and two of its respiration-impaired mutants. Both by direct plate counts of survivors and by quantitative ultraviolet spectrophotometric analyses of released cellular constituents, the respiration-impaired mutants were less vulnerable to the destructive actions of the basic proteins than were ordinary wild-type cells. The lethal incidence and the ultraviolet absorbing cellular substances released from wild-type cells by the proteins were markedly decreased in the presence of the oxidative phosphorylation uncouplers sodium azide, 2,4-dinitrophenol, and salicylanide and approximately equal to the effects produced on an oxidative phosphorylation mutant not treated with the uncouplers. The heightened resistance of a culture through mutational or chemical impairment of its respiratory system suggests a role of metabolic energy in the destructive action of various basic proteins on yeast cells.
对某些碱性蛋白质对白色念珠菌菌株及其两个呼吸受损突变体的破坏作用进行了比较研究。通过对存活菌的直接平板计数以及对释放的细胞成分进行定量紫外分光光度分析，发现呼吸受损突变体比普通野生型细胞对碱性蛋白质的破坏作用更不敏感。在存在氧化磷酸化解偶联剂叠氮化钠、2,4-二硝基苯酚和水杨酰胺的情况下，蛋白质对野生型细胞的致死率和释放的紫外吸收细胞物质明显降低，且与未用解偶联剂处理的氧化磷酸化突变体所产生的效应大致相同。通过突变或化学损伤其呼吸系统而使培养物的抗性增强，这表明代谢能量在各种碱性蛋白质对酵母细胞的破坏作用中发挥了作用。
