“衣被蛋白复合体”：一种胞质蛋白复合体，包含非网格蛋白包被的高尔基体运输小泡的亚基。

'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles.

作者信息

Waters M G, Serafini T, Rothman J E

机构信息

Department of Molecular Biology, Princeton University, New Jersey 08544.

出版信息

Nature. 1991 Jan 17;349(6306):248-51. doi: 10.1038/349248a0.

DOI:10.1038/349248a0

PMID:1898986

Abstract

Golgi-derived coated vesicles contain a set of coat proteins of relative molecular mass 160,000 (Mr 160K; alpha-COP), 110K (beta-COP), 98K (gamma-COP) and 61K (delta-COP), and several smaller subunits. We have now identified and purified a cytosolic complex containing the same four coat proteins as those of Golgi transport vesicles. We term this complex the Golgi coat promoter or 'coatomer'. The coatomer also contains polypeptides of Mr 36K, 35K and 20K. It represents about 0.2% of soluble cytosolic protein. Gel filtration of unfractionated cytosol indicates that beta-COP resides exclusively in the coatomer complex. The complex seems to be a likely candidate for the unassembled precursor of Golgi coated vesicles, and its purification should help investigations of the role of coat proteins in membrane budding, for which it is necessary to use a refined cell-free system.

摘要

源自高尔基体的被膜小泡含有一组相对分子质量分别为160,000（Mr 160K；α-COP）、110K（β-COP）、98K（γ-COP）和61K（δ-COP）的被膜蛋白，以及几个较小的亚基。我们现已鉴定并纯化出一种胞质复合物，它含有与高尔基体运输小泡相同的四种被膜蛋白。我们将这种复合物称为高尔基体被膜启动子或“被膜体”。被膜体还含有相对分子质量为36K、35K和20K的多肽。它约占可溶性胞质蛋白的0.2%。对未分级的胞质溶胶进行凝胶过滤表明，β-COP仅存在于被膜体复合物中。该复合物似乎是高尔基体被膜小泡未组装前体的一个可能候选物，其纯化有助于研究被膜蛋白在膜出芽中的作用，为此需要使用一种精细的无细胞系统。