土耳其安卡拉猫身上汉赛巴尔通体的调查。

Investigation of Bartonella henselae in cats in Ankara, Turkey.

作者信息

Celebi B, Kilic S, Aydin N, Tarhan G, Carhan A, Babur C

机构信息

Communicable Diseases Research Department, Refik Saydam National Hygiene Center, Ankara, Turkey.

出版信息

Zoonoses Public Health. 2009 May;56(4):169-75. doi: 10.1111/j.1863-2378.2008.01170.x. Epub 2008 Nov 4.

DOI:10.1111/j.1863-2378.2008.01170.x

PMID:18990198

Abstract

The purpose of this study was to determine Bartonella henselae prevalance in cats in Ankara. Whole bloods and sera collected from 256 cats were investigated for the presence feline Bartonella species by culture and sera were tested for the presence of antibodies against B. henselae IgG using immunofluorescence assay. Bartonella species were isolated by blood culture from 24 (9.4%) cats. Bartonella isolates were subjected to restriction fragment length polymorphism (RFLP) by using TaqI and HhaI endonucleases to identify species. Twenty-one isolates were determined as B. henselae and three of 24 isolates were determined as Bartonella clarridgeiae with RFLP. The bacteraemia prevalence and seroprevalence of B. henselae IgG antibodies in cats was detected as 8.2% and 18.6% respectively. This is the first report on B. henselea and B. clarridgeiae in cats in Turkey.

摘要

本研究的目的是确定安卡拉猫身上汉氏巴尔通体的流行情况。从256只猫采集全血和血清，通过培养调查猫巴尔通体属物种的存在情况，并使用免疫荧光测定法检测血清中抗汉氏巴尔通体IgG抗体的存在情况。通过血培养从24只（9.4%）猫中分离出巴尔通体属物种。使用TaqI和HhaI核酸内切酶对巴尔通体分离株进行限制性片段长度多态性（RFLP）分析以鉴定物种。通过RFLP分析，21株分离株被鉴定为汉氏巴尔通体，24株分离株中的3株被鉴定为克拉氏巴尔通体。检测到猫中汉氏巴尔通体IgG抗体的菌血症患病率和血清阳性率分别为8.2%和18.6%。这是土耳其关于猫身上汉氏巴尔通体和克拉氏巴尔通体的首次报告。