Institute of Bioengineering and Nanotechnology, Singapore.
J Neurovirol. 2008 Nov;14(6):563-8. doi: 10.1080/13550280802290606.
To prolong the transient gene expression mediated by baculoviral vector, the inverted terminal repeats and Rep gene of adeno-associated virus were incorporated into the genome of baculovirus, creating a hybrid baculovirus-adeno-associated viral (AAV) vector. By using previously constructed composite neuron-specific and astrocyte-specific promoters in this hybrid viral vector, sustained transgene expressions could be achieved in human neuronal and glial cell lines, but not in the correspondent rodent cell lines. This hybrid baculovirus-AAV vector might be useful for gene therapy of chronic neurological diseases in the central nervous system, such as Parkinson's disease and Alzheimer's disease.
为了延长杆状病毒载体介导的瞬时基因表达，将腺相关病毒的反向末端重复序列和 Rep 基因整合到杆状病毒基因组中，构建了一种杆状病毒-腺相关病毒（AAV）混合载体。利用该混合病毒载体中先前构建的复合神经元特异性和星形胶质细胞特异性启动子，可以在人神经元和神经胶质细胞系中实现持续的转基因表达，但不能在相应的啮齿动物细胞系中实现。这种杆状病毒-AAV 混合载体可能对治疗中枢神经系统慢性神经疾病（如帕金森病和阿尔茨海默病）的基因治疗有用。
