Lozza Facundo A, Chinchilla Leonardo A, Barbeito Claudio G, Goya Rodolfo G, Gimeno Eduardo J, Portiansky Enrique L
Institute of Pathology, School of Veterinary Sciences, National University of La Plata, La Plata, Argentina.
Neuropathology. 2009 Jun;29(3):258-62. doi: 10.1111/j.1440-1789.2008.00974.x. Epub 2008 Oct 20.
Aging is a process where histochemical changes occur. Some of these may consist of age-dependent loss of expression of some cell markers. Conversely, cell markers not expressed in young animals may be detectable in their older counterparts. Histochemical age changes in carbohydrate profiles in the spinal cord have not been documented. In order to fill this information gap lectin histochemistry and image analysis were used to characterize the histochemical age changes occurring in the cervical segments of the rat spinal cord. From a battery of 11 lectins, the more important age changes were detected with Glicine maximus (SBA)-lectin. Thus, SBA-lectin neuronal staining which was moderately positive in the cervical segments of young animals was negative in old rats. In contrast the same lectin which did not react with the ependyma of young animals strongly bound to the ependyma of senescent rats. None of the tested lectins bound to glial cells, either in young or old animals. In no case the senile animals evidenced anatomopathological changes. We conclude that although in the aged spinal cord changes in lectin histochemical binding patterns occur, they do not reflect a pathologic situation.
衰老过程中会发生组织化学变化。其中一些变化可能包括某些细胞标志物表达随年龄增长而丧失。相反，在幼龄动物中未表达的细胞标志物可能在老龄动物中被检测到。脊髓中碳水化合物谱的组织化学年龄变化尚未见报道。为了填补这一信息空白，采用凝集素组织化学和图像分析来表征大鼠脊髓颈段发生的组织化学年龄变化。从11种凝集素中，用大豆凝集素（SBA）检测到了更重要的年龄变化。因此，SBA凝集素在幼龄动物颈段呈中度阳性的神经元染色在老龄大鼠中为阴性。相反，同一种凝集素在幼龄动物的室管膜上无反应，但在老龄大鼠的室管膜上强烈结合。在幼龄或老龄动物中，所测试的凝集素均未与神经胶质细胞结合。在任何情况下，老龄动物均未出现解剖病理学变化。我们得出结论，虽然老龄脊髓中凝集素组织化学结合模式发生了变化，但它们并不反映病理情况。
