使用含有两性离子表面活性剂的洗脱液通过离子排斥/阳离子交换色谱法测定人唾液中的常见无机离子。

Ion-exclusion/cation-exchange chromatographic determination of common inorganic ions in human saliva by using an eluent containing zwitterionic surfactant.

作者信息

Mori Masanobu, Iwata Tomotaka, Satori Tatsuya, Ohira Shin-Ichi, Itabashi Hideyuki, Tanaka Kazuhiko

机构信息

Graduate School of Engineering, Gunma University, Kiryu, Gunma, Japan.

出版信息

J Chromatogr A. 2008 Dec 12;1213(2):125-9. doi: 10.1016/j.chroma.2008.08.001. Epub 2008 Aug 6.

DOI:10.1016/j.chroma.2008.08.001

PMID:18992886

Abstract

Ion-exclusion/cation-exchange chromatography with an eluent containing the bile salt-type zwitterionic surfactant CHAPS was performed in order to evaluate variations in anion (SO(4)(2-), NO(3)(-), and SCN(-)) and cation (Na(+), K(+), NH(4)(+), Mg(2+), and Ca(2+)) concentrations in human saliva. CHAPS prevents the adsorption of proteins to the stationary phase, i.e., weakly acidic cation-exchange resin, since it aggregates proteins without denaturing them. Addition of 1mM CHAPS to the eluent comprising 6mM tartaric acid and 7 mM 18-crown-6 yielded reproducible separations of anions and cations in protein-containing saliva. The resolutions of anions and cations were not significantly affected by the addition of CHAPS to the eluent. The concentrations of Na(+) and K(+) varied before and after meals; or that of SCN(-), upon smoking. The relative standard deviations of peak areas ranged from 0.3 to 5.1% in 1 day (n=20) and from 1.4 to 5.8% over 6 days (n=6).

摘要

为了评估人唾液中阴离子（SO(4)(2-)、NO(3)(-)和SCN(-)）和阳离子（Na(+)、K(+)、NH(4)(+)、Mg(2+)和Ca(2+)）浓度的变化，采用了离子排斥/阳离子交换色谱法，其洗脱液含有胆汁盐型两性离子表面活性剂CHAPS。CHAPS可防止蛋白质吸附到固定相上，即弱酸性阳离子交换树脂上，因为它能使蛋白质聚集而不使其变性。向含有6mM酒石酸和7mM 18-冠-6的洗脱液中添加1mM CHAPS，可实现含蛋白质唾液中阴离子和阳离子的可重复分离。向洗脱液中添加CHAPS对阴离子和阳离子的分离度没有显著影响。Na(+)和K(+)的浓度在饭前和饭后有所变化；而SCN(-)的浓度在吸烟时会发生变化。峰面积的相对标准偏差在1天内（n = 20）为0.3%至5.1%，在6天内（n = 6）为1.4%至5.8%。