Lee Jeremy, Tscheliessnig Anne, Chen Allen, Lee Yih Yean, Adduci Gloria, Choo Andre, Jungbauer Alois
Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, No. 06-01 Centros, Singapore 138668, Singapore.
J Chromatogr A. 2009 Mar 27;1216(13):2683-8. doi: 10.1016/j.chroma.2008.10.067. Epub 2008 Oct 21.
An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.
