Schuwerack P-M M, Lewis John W, Jones Peter
University of Plymouth at Britannia Royal Naval College, Dartmouth, Devon TQ6 9EN, UK.
Ecotoxicol Environ Saf. 2009 May;72(4):1220-7. doi: 10.1016/j.ecoenv.2008.07.004. Epub 2008 Nov 6.
Climatic and man-made impacts induced dynamic molecular responses in the South African freshwater crab, Potamonautes warreni. Adult crabs exhibited MT-like protein, binding Cd (0.02micromolg(-1) wet mass+/-0.02), Cu (0.326micromolg(-1) wet mass+/-0.15), and Zn (0.534micromolg(-1) wet mass+/-0.20). The native protein binding Cd, Cu, and Zn showed a respective molecular mass (M) of 9.10kDa+/-1.74, 8.95kDa+/-1.66, and 9.32kDa+/-0.93. With exposure to 0.2mgCd(2+)l(-1) for up to 21 days in 50% of these crabs approximately 90% of Cd was bound to the MT-like protein component (8.54kDa+/-1.64), coinciding with a Zn-bound MT-like component (8.2kDa+/-1.54). Less than 10% were bound in the high M protein component, suggesting a protective function of the protein. In the remaining crabs metals were bound to protein (6.8kDa) with a predominant Cu-binding component.
