Satoh K, Seki H, Sakamoto H
Faculty of Medicine, Department of Obstetrics and Gynecology, Nihon University, Tokyo, Japan.
Am J Kidney Dis. 1991 Feb;17(2):133-8. doi: 10.1016/s0272-6386(12)81117-6.
Enzymes involved in prostaglandin I2 (PGI2) and thromboxane A2 (TXA2) synthesis were studied in maternal and fetal platelets and venous endothelium from normotensive pregnant controls (n = 70), women with mild preeclampsia (MP, n = 45), and severe preeclampsia (SP, n = 34). Activities of phospholipase A2 (PHA2), cyclooxygenase (PGHS), and PGI2 synthetase (PGIS) or TXA2 synthetase (TXAS) were determined in platelets and in endothelial cells. The PGHS enzyme was studied further by immunoblot methodology. In maternal platelets: Vmax (per 10(-10) mol/mg protein) and Michaelis-Menten constant (Km) (10(-7) mol, mean +/- SEM) of PHA2 were 3.0 +/- 0.8, 3.0 +/- 0.7, and 31.7 +/- 10.9* maximum velocity (Vmax) and 1.8 +/- 0.3, 2.0 +/- 0.8, and 0.8 +/- 0.2 (Km) in normal control (NC), mild preeclampsia (MP), and severe preeclampsia (SP), respectively (P less than 0.05 against NC). The apparent overall PGHS plus TXAS activity was 10.2 +/- 1.8, 23.8 +/- 7.1, and 68.8 +/- 18.8 (Vmax) and 3.2 +/- 1.3, 5.4 +/- 1.4, and 6.9 +/- 1.2* (Km, P less than 0.05 against NC). TXA synthesis in fetal platelets demonstrated PHA2 activity of 6.4 +/- 1.4, 12.0 +/- 1.3, and 17.2 +/- 3.2 (Vmax) and 3.5 +/- 0.9, 2.2 +/- 1.5, and 0.7 +/- 0.3* (Km, P less than 0.05 against NC), respectively, whereas an apparent overall PGHS plus TXAS activity was 18.5 +/- 2.8, 87.5 +/- 12.5, and 3.6 +/- 0.1* (Vmax) and 4.8 +/- 1.0, 8.8 +/- 1.2, and 0.8 +/- 0.3* (Km, *P less than 0.05 against NC).(ABSTRACT TRUNCATED AT 250 WORDS)
对血压正常的孕妇对照组（n = 70）、轻度子痫前期孕妇（MP，n = 45）和重度子痫前期孕妇（SP，n = 34）的母血和胎儿血小板以及静脉内皮中参与前列腺素I2（PGI2）和血栓素A2（TXA2）合成的酶进行了研究。测定了血小板和内皮细胞中磷脂酶A2（PHA2）、环氧化酶（PGHS）以及PGI2合成酶（PGIS）或TXA2合成酶（TXAS）的活性。通过免疫印迹法进一步研究了PGHS酶。在母血血小板中：PHA2的最大反应速度（Vmax，每10^(-10) mol/毫克蛋白）和米氏常数（Km）（10^(-7) mol，平均值±标准误），正常对照组（NC）分别为3.0±0.8、3.0±0.7以及31.7±10.9最大反应速度（Vmax）和1.8±0.3、2.0±0.8以及0.8±0.2（Km），轻度子痫前期组（MP）分别为31.7±10.9最大反应速度（Vmax）和1.8±0.3、2.0±0.8以及0.8±0.2（Km），重度子痫前期组（SP）分别为31.7±10.9最大反应速度（Vmax）和1.8±0.3、2.0±0.8以及0.8±0.2（Km），（与NC相比，P<0.05）。PGHS加TXAS的表观总活性，Vmax分别为10.2±1.8、23.8±7.1以及68.8±18.8，Km分别为3.2±1.3、5.4±1.4以及6.9±1.2（与NC相比，P<0.05）。胎儿血小板中的TXA合成显示PHA2活性，Vmax分别为6.4±1.4、12.0±1.3以及17.2±3.2，Km分别为3.5±0.9、2.2±1.5以及0.7±0.3*（与NC相比，P<0.05），而PGHS加TXAS的表观总活性，Vmax分别为18.5±2.8、87.5±12.5以及3.6±0.1*，Km分别为4.8±1.0、8.8±1.2以及0.8±0.3*（*与NC相比，P<0.05）。（摘要截选至250词）
