Mileva Gerri, Zysman Daniel, Groothuis Sally, Lewis John E
Department of Biology, University of Ottawa, Ottawa, ON, Canada.
J Physiol Paris. 2008 Jul-Nov;102(4-6):173-80. doi: 10.1016/j.jphysparis.2008.10.012. Epub 2008 Oct 17.
Electrosensory systems comprise extensive feedback pathways. It is also well known that these pathways exhibit synaptic plasticity on a wide-range of time scales. Recent in vitro brain slice studies have characterized synaptic plasticity in the two main feedback pathways to the electrosensory lateral line lobe (ELL), a primary electrosensory nucleus in Apteronotus leptorhynchus. Currently-used slice preparations, involving networks in open-loop conditions, allow feedback inputs to be studied in isolation, a critical step in determining their synaptic properties. However, to fully understand electrosensory processing, we must understand how dynamic feedback modulates neuronal responses under closed-loop conditions. To bridge the gap between current in vitro approaches and more complex in vivo work, we present two new in vitro approaches for studying the roles of closed-loop feedback in electrosensory processing. The first involves a hybrid-network approach using dynamic clamp, and the second involves a new slice preparation that preserves one of the feedback pathways to ELL in a closed-loop condition.
电感应系统包含广泛的反馈通路。众所周知，这些通路在广泛的时间尺度上表现出突触可塑性。最近的体外脑片研究已经对电感应侧线叶（ELL）（线纹长背电鳗的一个主要电感应核）的两条主要反馈通路中的突触可塑性进行了表征。目前使用的脑片制备方法涉及开环条件下的网络，使得能够单独研究反馈输入，这是确定其突触特性的关键步骤。然而，为了全面理解电感应处理过程，我们必须了解动态反馈如何在闭环条件下调节神经元反应。为了弥合当前体外研究方法与更复杂的体内研究之间的差距，我们提出了两种新的体外方法来研究闭环反馈在电感应处理中的作用。第一种方法涉及使用动态钳位的混合网络方法，第二种方法涉及一种新的脑片制备方法，该方法在闭环条件下保留了一条通向ELL的反馈通路。
