质子化二茂铁-DNA嵌入的电化学和光谱研究。
Electrochemical and spectroscopic investigations of protonated ferrocene-DNA intercalation.
作者信息
Shah Afzal, Qureshi Rumana, Janjua Naveed Kausar, Haque Sayedul, Ahmad Safeer
机构信息
Department of Chemistry, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
出版信息
Anal Sci. 2008;24(11):1437-41. doi: 10.2116/analsci.24.1437.
The interaction of protonated ferrocene (PF) with chicken blood DNA (CB-DNA) has been investigated in vitro by cyclic voltammetry (CV) and UV-Vis spectroscopy as well as viscosity measurements under stomach pH and body temperature. The peak potentials shift in CV, hyperchromism in UV absorption titration, an increase in the viscosity of DNA and the results of the effect of ionic strength on the binding constant strongly support the intercalation of PF into the DNA double helix. The diffusion coefficients of PF in the presence and absence of DNA were 9.54 x 10(-11) and 1.34 x 10(-10) m2/s, respectively. The binding constant of the PF-DNA complex and the number of binding sites on a DNA molecule were calculated as being 3.07 x 10(2) M(-1) and 2.96, with the help of the Scatchard equation. An expression by Carter et al. was used for determining the binding site size (0.17 bp). The binding constant was also determined by UV absorption titration.
在体外，通过循环伏安法（CV）、紫外可见光谱以及在胃pH值和体温条件下的粘度测量，研究了质子化二茂铁（PF）与鸡血DNA（CB-DNA）的相互作用。CV中的峰电位移动、紫外吸收滴定中的增色现象、DNA粘度的增加以及离子强度对结合常数影响的结果，有力地支持了PF插入DNA双螺旋结构。有DNA和无DNA存在时PF的扩散系数分别为9.54×10⁻¹¹和1.34×10⁻¹⁰ m²/s。借助Scatchard方程，计算出PF-DNA复合物的结合常数和DNA分子上的结合位点数分别为3.07×10² M⁻¹和2.96。使用Carter等人的表达式来确定结合位点大小（0.17 bp）。结合常数也通过紫外吸收滴定法测定。
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