Liu Lan-Jun, Suzuki Takako, Tsunemitsu Hiroshi, Kataoka Michiyo, Ngata Noriyo, Takeda Naokazu, Wakita Takaji, Miyamura Tatsuo, Li Tian-Cheng
Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan.
Arch Virol. 2008;153(12):2291-5. doi: 10.1007/s00705-008-0248-x. Epub 2008 Nov 9.
The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm(3) and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 10(7) Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity.
利用重组杆状病毒和昆虫Tn5细胞表达猪圆环病毒2型（PCV2）的衣壳蛋白。大量28 kDa的蛋白释放到培养基中，并自组装成类似PCV2的颗粒（PCV2-LPs），其浮力密度为1.365 g/cm³，直径为20 nm。PCV2-LPs高效表达，每10⁷个Tn5细胞可产生1 mg纯化颗粒。PCV2-LPs具有与天然PCV2颗粒相似的抗原性，这使我们能够开发一种灵敏检测PCV2特异性IgG抗体的方法。此外，由于其强大的免疫原性，PCV2-LPs似乎是最有前景的PCV2疫苗候选物。
