Lippiat Jonathan D
Institute of Membrane and Systems Biology, University of Leeds, UK.
Methods Mol Biol. 2008;491:141-9. doi: 10.1007/978-1-59745-526-8_11.
Many ion channels, particularly potassium channels, are regulated by intracellular substances, such as nucleotides or Ca(2+). These modulators are washed out of the cell during standard whole-cell patch clamp recordings, or maintained at a particular concentration if they are included in the pipette solution. Perforated patch clamp recording permits electrical access between the cell and the patch pipette using pore-forming antibiotics such as nystatin or amphotericin B. These are permeable to small monovalent ions but present a physical barrier to the larger impermeable ions and molecules. This maintains the integrity of many cytoplasmic components including soluble second messengers, and also helps to prevent channel "run down".
许多离子通道，尤其是钾通道，受细胞内物质如核苷酸或Ca(2+)的调节。在标准的全细胞膜片钳记录过程中，这些调节剂会被洗出细胞，或者如果将它们包含在微电极溶液中，则可维持在特定浓度。穿孔膜片钳记录允许使用制霉菌素或两性霉素B等成孔抗生素在细胞和膜片微电极之间实现电连接。这些抗生素对单价小离子具有通透性，但对较大的不可通透离子和分子构成物理屏障。这维持了包括可溶性第二信使在内的许多细胞质成分的完整性，也有助于防止通道“功能丧失”。
