蓝细菌中正常隐秘的psbA基因的低氧诱导。

Low-oxygen induction of normally cryptic psbA genes in cyanobacteria.

作者信息

Summerfield Tina C, Toepel Jörg, Sherman Louis A

机构信息

Department of Biological Sciences, Purdue University, 201 South University Street, Hansen Hall, West Lafayette, Indiana 47907, USA.

出版信息

Biochemistry. 2008 Dec 9;47(49):12939-41. doi: 10.1021/bi8018916.

DOI:10.1021/bi8018916

PMID:18998707

Abstract

Microarray analysis indicated low-O(2) conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1' protein of photosystem II in Synechocystis sp. PCC 6803. Using a DeltapsbA2:DeltapsbA3 strain, we show the psbA1 transcript is translated and the resultant D1' is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron-sulfur protein. We conclude this cluster may represent an important adaptation to changing O(2) levels that cyanobacteria experience.

摘要

微阵列分析表明，低氧条件导致集胞藻PCC 6803中psbA1上调，psbA1是通常低丰度的转录本，编码光系统II的D1'蛋白。使用DeltapsbA2:DeltapsbA3菌株，我们发现psbA1转录本被翻译，产生的D1'被插入到功能性PSII复合物中。另外两种蓝藻菌株具有在低氧条件下被诱导的psbA基因。在所检测的三个菌株中的两个菌株中，psbA是一个上调基因簇的一部分，该基因簇包括一个替代型铁硫蛋白。我们得出结论，该基因簇可能代表了蓝藻所经历的氧气水平变化的一种重要适应性。