Khan S P, Raza A, Barcos M, Yousuf N, Saikia T, Masterson M, Bennett J, Browman G, Goldberg J, Grunwald H
Roswell Park Memorial Institute, Buffalo, NY.
Leuk Res. 1991;15(1):51-7. doi: 10.1016/0145-2126(91)90144-i.
Presence of megakaryocytic cells in patients with myeloid disorders were investigated by staining plastic embedded biopsy sections with an anti-Factor VIII antibody (AFA). Two hundred and fifty cases were studied, 207 of whom had acute myeloid leukemia (AML) while 43 had myelodysplastic syndromes (MDS). Abnormal clusters of AFA positive cells indicating multilineage disease were identified in 17% with primary AML (30/175), 38% with secondary AML (12/32) and 42% cases of MDS (18/43). Biological characteristics of these 60 AFA positive cases were investigated. No unique differences in cell cycle characteristics following bromodeoxyuridine (BrdU) were identified. We confirm several recent reports that the incidence of multilineage involvement in AML is substantial.
通过用抗VIII因子抗体（AFA）对塑料包埋活检切片进行染色，研究了骨髓疾病患者中巨核细胞的存在情况。共研究了250例病例，其中207例患有急性髓系白血病（AML），43例患有骨髓增生异常综合征（MDS）。在原发性AML患者中，17%（30/175）发现AFA阳性细胞异常聚集，提示多系疾病；继发性AML患者中为38%（12/32），MDS患者中为42%（18/43）。对这60例AFA阳性病例的生物学特征进行了研究。未发现溴脱氧尿苷（BrdU）处理后细胞周期特征存在独特差异。我们证实了最近的几份报告，即AML中多系受累的发生率相当高。
