Chaudhry Uzma A, Dore Sylvain
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Am J Alzheimers Dis Other Demen. 2009 Feb-Mar;24(1):46-51. doi: 10.1177/1533317508323655. Epub 2008 Nov 10.
Anti-inflammatory drugs reduce the risk of Alzheimer's disease but fail to slow its progression. Studying the expression of prostaglandin E(2) synthases downstream of cyclooxygenase-2 is important. Here, the expression patterns of cytosolic prostaglandin E( 2) synthases, an immediate prostaglandin E(2) source was investigated. Sections taken from the middle frontal gyrus of brains of 10 patients with Alzheimer's and 5 age-matched controls were examined by immunostaining for the presence of the synthases. Immunofluorescence analysis of control brains showed that cytosolic prostaglandin E(2) synthases co-localize with microglia, neurons, and endothelium markers, but not with astrocytes or smooth muscle cells. Immunohistochemical staining for the synthases was positive in the pyramidal neurons of controls but barely detectable in the brain of Alzheimer's patients. These findings revealed that cytosolic prostaglandin E(2) synthases is found in microglia, neurons, and endothelium of control human middle frontal gyrus and that its levels decrease in pyramidal cells of Alzheimer's disease brains.
抗炎药物可降低患阿尔茨海默病的风险，但无法减缓其病程进展。研究环氧化酶-2下游的前列腺素E(2)合成酶的表达具有重要意义。在此，对胞质前列腺素E(2)合成酶的表达模式进行了研究，其是前列腺素E(2)的直接来源。对取自10例阿尔茨海默病患者及5例年龄匹配对照者大脑额中回的切片进行免疫染色，以检测合成酶的存在情况。对照大脑的免疫荧光分析显示，胞质前列腺素E(2)合成酶与小胶质细胞、神经元和内皮细胞标志物共定位，但不与星形胶质细胞或平滑肌细胞共定位。合成酶的免疫组化染色在对照者的锥体细胞中呈阳性，但在阿尔茨海默病患者的大脑中几乎检测不到。这些发现表明，在对照人额中回的小胶质细胞、神经元和内皮细胞中可发现胞质前列腺素E(2)合成酶，且其在阿尔茨海默病大脑锥体细胞中的水平降低。
