Shin Jae-Ho, Heo Gun-Young, Kelman Zvi
Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Daegu 702-701, Republic of Korea and the University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, Rockville, Maryland 20850.
Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Daegu 702-701, Republic of Korea and the University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, Rockville, Maryland 20850.
J Biol Chem. 2009 Jan 2;284(1):540-546. doi: 10.1074/jbc.M806803200. Epub 2008 Nov 10.
The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in archaea and eukarya. The structure of the single MCM protein homologue from the archaeon Methanothermobacter thermautotrophicus is not yet clear, and hexameric, heptameric, octameric, and dodecameric structures, open rings, and filamentous structures have been reported. Using a combination of biochemical and structural analysis, it is shown here that the M. thermautotrophicus MCM helicase is active as a hexamer.
微小染色体维持（MCM）复合物被认为在古细菌和真核生物中作为复制解旋酶发挥作用。来自嗜热自养甲烷杆菌的单个MCM蛋白同源物的结构尚不清楚，并且已经报道了六聚体、七聚体、八聚体和十二聚体结构、开环结构和丝状结构。本文通过生化分析和结构分析相结合的方法表明，嗜热自养甲烷杆菌MCM解旋酶以六聚体形式具有活性。
