Tang Ke, Cao Li, Fan Song-qing, Wu Ming-hua, Huang He, Zhou Yan-hong, Zhou Ming, Tang Yun-lian, Wang Rong, Zeng Fang, Liao Ping, Li Xiao-ling, Li Gui-yuan
Cancer Research Institute, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Oct;33(10):892-7.
To explore the effect of all-trans-retinoic acid (ATRA) on the growth inhibition and cellular differentiation of C6 glioma cells.
Human glioma C6 cells were treated with 5 mg/L ATRA,and the inhibition of cell growth was assessed by methyl thiazolyl tetrazolium assay. The differentiation of C6 cells was determined by flow cytometry, microscopy,transmission electron microscope, and immunohistochemical technique.
Treatment of ATRA could result in the growth inhibition of C6 cells, and the cell density significantly decreased(P<0.01). The cell cycle distribution was changed, G0/G1 phase was prolonged, and cells at S phase decreased(P<0.01). The C6 glioma cells displayed normal fibroblast-like morphology under the microscope before the induction, and the ATRA-treated C6 cells became slightly long, turned into round in the middle, and had protrusions at both ends. The ATRA-treated C6 cells did not display obvious apoptosis by flow cytometry(P>0.05).Whereas, early apoptosis was observed under the transmission electron microscope, the vacuoles increased, the mitochondria and endoplasmic reticulum were abundant in the cytoplasm, and the cellular structures tended to be normal.The expression of glial fibrillaryacidic protein in C6 cells increased in the treatment group.
ATRA can inhibit the proliferation, and induce the differentiation of C6 glioma cells.
探讨全反式维甲酸（ATRA）对C6胶质瘤细胞生长抑制及细胞分化的影响。
用5mg/L ATRA处理人胶质瘤C6细胞，采用甲基噻唑基四氮唑蓝法评估细胞生长抑制情况。通过流式细胞术、显微镜、透射电子显微镜及免疫组织化学技术检测C6细胞的分化情况。
ATRA处理可导致C6细胞生长抑制，细胞密度显著降低（P<0.01）。细胞周期分布改变，G0/G1期延长，S期细胞减少（P<0.01）。诱导前显微镜下C6胶质瘤细胞呈正常成纤维细胞样形态，经ATRA处理的C6细胞变长，中部变圆，两端有突起。流式细胞术检测显示经ATRA处理的C6细胞未出现明显凋亡（P>0.05）。然而，透射电子显微镜下观察到早期凋亡，空泡增多，细胞质中线粒体和内质网丰富，细胞结构趋于正常。治疗组C6细胞中胶质纤维酸性蛋白表达增加。
ATRA可抑制C6胶质瘤细胞增殖并诱导其分化。
