Kawasaki Masako, Anzawa Kazushi, Wakasa Asako, Takeda Kiminobu, Tanabe Hiroshi, Mochizuki Takashi, Ishizaki Hiroshi, Hemashettar Basavaraj M
Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Nihon Ishinkin Gakkai Zasshi. 2008;49(4):311-8. doi: 10.3314/jjmm.49.311.
To determine the relationships among Trichophyton species we constructed three phylogenies, based on the nucleotide sequences of the actin, rRNA and DNA topoisomerase II genes. These phylogenies showed several conflicting branch points. For example, strains of T. verrucosum,T. concentricum and T. mentagrophytes var. erinacei were mingled with strains of Arthroderma benhamiae and could not be separated into their own phylogenic groups. In addition, strains of A. vanbreuseghemii, T. tonsurans, T. mentagrophytes var. interdigitale and T. mentagrophytes var. quinckeanum were mingled with strains of A. simii and could not be separated into their own phylogenic groups. T. rubrum and T. violaceum made up a clade, which was phylogenetically related to the A. benhamiae clade or A. simii clade, depending on the gene examined. These findings indicate the need to reevaluate the boundaries among Trichophyton species using an alternative to morphological or molecular biological methods.
为了确定毛癣菌属各物种之间的关系，我们基于肌动蛋白、rRNA和DNA拓扑异构酶II基因的核苷酸序列构建了三个系统发育树。这些系统发育树显示出几个相互冲突的分支点。例如，疣状毛癣菌、同心毛癣菌和须癣毛癣菌变种erinacei的菌株与本汉节皮菌的菌株混在一起，无法分成各自的系统发育组。此外，万氏节皮菌、断发毛癣菌、指间毛癣菌变种和昆克氏毛癣菌变种的菌株与西氏节皮菌的菌株混在一起，无法分成各自的系统发育组。红色毛癣菌和紫色毛癣菌组成一个进化枝，根据所检测的基因，该进化枝在系统发育上与本汉节皮菌进化枝或西氏节皮菌进化枝相关。这些发现表明，需要使用形态学或分子生物学方法以外的方法重新评估毛癣菌属各物种之间的界限。
