Algin Mustafa Cem, Hacioglu Alper, Yaylak Faik, Gulcan Erim, Aydin Tayfun, Hacioglu Buket Altunkara, Ilhan Demet, Cevik Arif Alper, Ates Ersin
Department of General Surgery, Kutahya, Turkey.
Adv Ther. 2008 Dec;25(12):1353-74. doi: 10.1007/s12325-008-0114-y.
The aim of the present study was to evaluate the role of erythropoietin (EPO) in liver and renal injury following hemorrhagic shock (HS) after inhibition of tyrosine kinase activity in rats..
Forty-eight Sprague-Dawley rats were assigned to six groups: (I) HS alone; (II) HS followed by retransfusion; (III) EPO and genistein followed by HS; (IV) EPO and genistein followed by HS, followed by retransfusion; (V) HS followed by EPO and genistein; and (VI) HS followed by EPO and genistein, followed by retransfusion. HS was induced for 60 minutes after withdrawal of 30% of the calculated total blood volume of each rat from the left femoral artery. Blood and tissue samples (from the kidney and liver) were obtained 60 minutes after HS in Group I, III, and V; blood and tissue samples were obtained 60 minutes after retransfusion in Group II, IV, and VI. In Group III and IV, EPO was given 60 minutes before HS, and genistein 30 minutes before HS. In Group V and VI, EPO and genistein were given 30 minutes after HS.
Liver and renal injury were significantly attenuated with EPO and genistein administration.
These results suggest that EPO is effective in attenuating liver and renal injury in HS, even with inhibition of tyrosine kinase activity with genistein.
本研究旨在评估在大鼠酪氨酸激酶活性受到抑制后,促红细胞生成素(EPO)在失血性休克(HS)后肝损伤和肾损伤中的作用。
将48只Sprague-Dawley大鼠分为六组:(I)单纯失血性休克组;(II)失血性休克后再输血组;(III)EPO和染料木黄酮预处理后失血性休克组;(IV)EPO和染料木黄酮预处理后失血性休克,再输血组;(V)失血性休克后给予EPO和染料木黄酮组;(VI)失血性休克后给予EPO和染料木黄酮,再输血组。从每只大鼠左股动脉抽取计算出的总血量的30%后诱导失血性休克60分钟。I组、III组和V组在失血性休克60分钟后采集血液和组织样本(来自肾脏和肝脏);II组、IV组和VI组在再输血60分钟后采集血液和组织样本。在III组和IV组中,在失血性休克前60分钟给予EPO,在失血性休克前30分钟给予染料木黄酮。在V组和VI组中,在失血性休克后30分钟给予EPO和染料木黄酮。
给予EPO和染料木黄酮后,肝损伤和肾损伤明显减轻。
这些结果表明,即使使用染料木黄酮抑制酪氨酸激酶活性,EPO在减轻失血性休克中的肝损伤和肾损伤方面仍有效。