来自野驴、斑马和汤氏瞪羚的马疱疹病毒1型分离株的分子系统发育研究

Molecular phylogeny of equine herpesvirus 1 isolates from onager, zebra and Thomson's gazelle.

作者信息

Ghanem Y M, Fukushi H, Ibrahim E S M, Ohya K, Yamaguchi T, Kennedy M

机构信息

Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.

出版信息

Arch Virol. 2008;153(12):2297-302. doi: 10.1007/s00705-008-0247-y. Epub 2008 Nov 11.

DOI:10.1007/s00705-008-0247-y

PMID:19002555

Abstract

Viruses related to equine herpesvirus type 1 (EHV-1) were isolated from an aborted fetus of an onager (Equus hemionus) in 1984, an aborted fetus of Grevy's zebra (Equus grevyi) in 1984 and a Thomson's gazelle (Gazella thomsoni) with nonsuppurative encephalitis in 1996, all in the USA. The mother of the onager fetus and the gazelle were kept near plains zebras (Equus burchelli). In phylogenetic trees based on the nucleotide sequences of the genes for glycoproteins B (gB), I (gI), and E (gE), and teguments including ORF8 (UL51), ORF15 (UL45), and ORF68 (US2), the onager, Grevy's zebra and gazelle isolates formed a genetic group that was different from several horse EHV-1 isolates. Within this group, the onager and gazelle isolates were closely related, while the Grevy's zebra isolate was distantly related to these two isolates. The epizootiological origin of the viruses is discussed.

摘要

1984年，从美国一头藏野驴（Equus hemionus）的流产胎儿、一头细纹斑马（Equus grevyi）的流产胎儿以及1996年一头患有非化脓性脑炎的汤氏瞪羚（Gazella thomsoni）中分离出了与1型马疱疹病毒（EHV-1）相关的病毒。藏野驴胎儿的母亲和瞪羚被饲养在平原斑马（Equus burchelli）附近。在基于糖蛋白B（gB）、I（gI）和E（gE）以及包括开放阅读框8（ORF8，UL51）、开放阅读框15（ORF15，UL45）和开放阅读框68（US2）的被膜蛋白基因核苷酸序列构建的系统发育树中，藏野驴、细纹斑马和瞪羚的分离株形成了一个与几种马EHV-1分离株不同的遗传组。在这个组内，藏野驴和瞪羚的分离株密切相关，而细纹斑马的分离株与这两个分离株关系较远。文中讨论了这些病毒的流行病学起源。