通过激光扫描荧光显微镜检测A431细胞基底外侧膜中的表皮生长因子受体和E-钙黏蛋白。
Detection of epidermal growth factor receptors and E-cadherins in the basolateral membrane of A431 cells by laser scanning fluorescence microscopy.
作者信息
Fukuyama R, Shimizu N
机构信息
Department of Molecular Biology, Keio University School of Medicine, Tokyo.
出版信息
Jpn J Cancer Res. 1991 Jan;82(1):8-11. doi: 10.1111/j.1349-7006.1991.tb01737.x.
Abstract
We have examined the localization of epidermal growth factor (EGF) receptors and E-cadherins in cultured A431 cells, an epidermoid carcinoma cell line, using a laser scanning fluorescence microscope. The fluorescence signals generated by monoclonal antibodies against EGF receptor and E-cadherin were localized mainly in the cell-cell contact sites, about 3 microns in width. When these areas for cell adhesion were scanned perpendicularly, fluorescence was detected from a point 4 microns from the cell base for a distance of 8 microns towards the cell surface (about 16 microns from the cell base). These data suggest the subcellular localization of EGF receptors and E-cadherins in the basolateral membrane of A431 cells.
摘要
我们使用激光扫描荧光显微镜，研究了表皮生长因子（EGF）受体和E-钙黏蛋白在培养的表皮样癌细胞系A431细胞中的定位。抗EGF受体和E-钙黏蛋白的单克隆抗体产生的荧光信号主要定位在细胞间接触部位，宽度约为3微米。当垂直扫描这些细胞黏附区域时，从距离细胞基部4微米的点开始，朝着细胞表面检测到8微米距离（距细胞基部约16微米）的荧光。这些数据表明EGF受体和E-钙黏蛋白在A431细胞的基底外侧膜中的亚细胞定位。
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