在肌母细胞系 C2C12 分化过程中，鼠钙蛋白酶抑制蛋白同工型的持续表达。
Constant expression of mouse calpastatin isoforms during differentiation in myoblast cell line, C2C12.
机构信息
Lab of Molecular and Cellular Regulation, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.
出版信息
Cytotechnology. 2000 Jul;33(1-3):63-70. doi: 10.1023/A:1008194002923.
C2C12 is a myoblast cell line which is used to studydifferentiation into multinucleated cells in vitro. Addition of calpain inhibitors, calpeptin orE-64d, to the culture medium prevented the myoblasticfusion of C2C12 cells. Immunoblot studies usingaffinity-purified antibody, revealed that the expressedlevels of mouse calpastatin remained unaltered duringC2C12 cell fusion. The detected calpastatin migratedas a protein of 130 kDa on SDS-polyacrylamide gelelectrophoresis. The estimated molecular mass wassomewhat greater than that in mouse liver anderythrocytes, and much greater than that reported inrat myoblasts. The 130 kDa isoform may contain anadditional N-terminal region designated XL domainfound in bovine calpastatin.
C2C12 是一种成肌细胞系，用于研究体外的多核细胞分化。在培养基中添加钙蛋白酶抑制剂 calpeptin 或 E-64d 可以防止 C2C12 细胞的成肌融合。使用亲和纯化抗体的免疫印迹研究表明，在 C2C12 细胞融合过程中，表达的鼠钙蛋白酶抑制剂的水平保持不变。检测到的钙蛋白酶抑制剂在 SDS-聚丙烯酰胺凝胶电泳中迁移为 130 kDa 的蛋白质。估计的分子量比在鼠肝和红细胞中的稍大，比在大鼠成肌细胞中报道的大得多。130 kDa 同工型可能含有一个额外的 N 端区域，称为 XL 结构域，在牛钙蛋白酶抑制剂中发现。
Zotero 插件