Laboratory of Animal Reproductive Physiology, Animal Resource Science Section, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University, Fukuoka, 812-8581, Japan.
Cytotechnology. 2000 Jul;33(1-3):101-8. doi: 10.1023/A:1008167427746.
Chicken primordial germ cells (PGCs) collected from thecirculating blood in embryonic vessels at stage 13-15 were inter-embryonically, homo- or hetero-sexually,transferred to the blood vessels of recipient embryosat the same stage of development. Approximately 30%of the embryos treated with hetero-sexual transfer of PGCs had abnormal gonads, showing ovotestis likeorgans. In this case, some of these reversed gonadswere considered to be dependent upon the ratio of thenumber of PGCs from donor to recipient embryos. Oneof the treated embryos possessed completely reversedorgans. Therefore, the introduction of exogenousembryonic vessels was thought to be also useful forproducing transgened gonads.
鸡原始生殖细胞（PGCs）从胚胎 13-15 期的循环血管中收集，通过胚胎间、同性别或异性别转移到相同发育阶段的受体胚胎血管中。大约 30%用异性别 PGC 转移处理的胚胎出现异常性腺，表现出卵睾样器官。在这种情况下，一些逆转的性腺被认为取决于供体和受体胚胎中 PGC 数量的比例。其中一个处理胚胎具有完全逆转的器官。因此，引入外源性胚胎血管也被认为有助于产生转基因性腺。
